Gene-expression profiling of titanium-cell interaction
김창수, 신상완, 류재준, 황정원, 손성화, 김기남, 김명곤,
소속 상세정보
김창수 ( Kim Chang-Su ) - 분당서울대학교병원 치과보철과
신상완 ( Shin Sang-Wan ) - 고려대학교 의과대학 치과학교실
류재준 ( Ryu Jae-Jun ) - 고려대학교 의과대학 치과학교실
황정원 ( Hwang Jung-Won ) - 분당서울대학교병원 치과보철과
손성화 ( Sohn Sung-Hwa ) - 고려대학교 의과대학 생화학교실
김기남 ( Kim Ki-Nam ) - 고려대학교 의과대학 생화학교실
김명곤 ( Kim Meyoung-Kon ) - 고려대학교 의과대학 생화학교실
KMID : 0362420050430030393
Abstract
Statement of problem: In the process of bone formation, titanium (Ti) surface roughness is an important factor modulating osteoblastic function.
Purpose: This study was carried out to determine the effect of different Ti surface on biologic responses of a human osteoblast-like cell line (MG63).
Materials & Methods: MG63 cells were cultured on S (smooth), SLA (sandblasted largegrit and acid etching), HA (hydroxyapatite) Ti. The morphology and attachment of the cells were examined by SEM. The cDNAs prepared from total RNAs of MG63 were hybridized to a human cDNA microarray (1,152 elements).
Results: The appearances of the surfaces observed with SEM were different in the three types of dental substrates. The surface of SLA and HA were shown to be rougher than S. MG63 cells cultured on SLA and HA were cell-matrix interaction. In the expression of genes involved in osseointegration, upregulated genes were bone morphogenetic protein, Villin, Integrin, Insulin-like growth factors in different surfaces. Downregulated genes were fibroblast growth factor receptor 4, Bcl 2-related protein, collagen, CD4 in different surfaces.
Conclusion: The attachment and expression of key osteogenic regulatory genes were enhanced by surface roughness of the dental materials.
키워드
Titanium surfaces;expression profiling;cDNA microarray;MG63
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